Celowe zatrucie 48 ptaków drapieżnych – oznaczenie bendiokarbu metodą UHPLC-ESI-MS/MS – śmiertelny przypadek w Europie Wschodniej
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RIS BIB ENDNOTESimultaneous poisoning of 48 birds of prey – bendiocarb determination with the use of UHPLC-ESI-MS/MS method in fatal case from Eastern Europe
Data publikacji: 11.2022
Archiwum Medycyny Sądowej i Kryminologii, 2022, Vol. 72 (2), s. 67 - 80
https://doi.org/10.4467/16891716AMSIK.22.009.16807Autorzy
Simultaneous poisoning of 48 birds of prey – bendiocarb determination with the use of UHPLC-ESI-MS/MS method in fatal case from Eastern Europe
Aim: Bendiocarb is used against a wide range of insects but has already been withdrawn from the market in some countries. It poses a high risk to birds as they can accidentally ingest it while searching for food, followed by toxic effects. This paper presents the results of toxicological and histopathological studies of 48 cases of intentional birds of prey poisoning with bendiocarb in Eastern Europe, specifically Poland.
Materials and methods: A novel ultra-high-performance liquid chromatography-triple quadrupole-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for bendiocarb determination in animal liver samples was developed and fully validated. The sample preparation technique was based on one-step precipitation of proteins with cold acetonitrile. The internal standard used was carbaryl-d7. Full time of analysis was less than 10 minutes. The application of the UHPLC-ESI-MS/MS method allowed us to achieve the lowest LOQ (1 ng/g) of bendiocarb in biological samples to date.
Results: Necropsies and histopathological examinations of common ravens (Corvus corax), western marsh harriers (Circus aeruginosus), red kites (Milvus milvus), and a white-tailed eagle (Haliaeetus albicilla) revealed multi-organ toxicity manifested as congestion, oedema, or stagnation of blood. An analytical investigation confirmed the presence of bendiocarb in liver in the 1808–7721 ng/g range. Furthermore, the presence of this compound was qualitatively confirmed in the stomach and beak contents and also in the bait located near the deceased animals.
Conclusions: A comprehensive forensic examination is crucial to monitor wildlife fatalities, especially applying a combined analytical and histopathological approach to identify and eliminate highly toxic substances which pose a threat to the ecosystem.
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Informacje: Archiwum Medycyny Sądowej i Kryminologii, 2022, Vol. 72 (2), s. 67 - 80
Typ artykułu: Oryginalny artykuł naukowy
Tytuły:
Simultaneous poisoning of 48 birds of prey – bendiocarb determination with the use of UHPLC-ESI-MS/MS method in fatal case from Eastern Europe
Celowe zatrucie 48 ptaków drapieżnych – oznaczenie bendiokarbu metodą UHPLC-ESI-MS/MS – śmiertelny przypadek w Europie Wschodniej
Institute of Toxicology Research, Borowa, Poland
Department of Forensic Medicine, Wroclaw Medical University, Poland
Medical University of Lublin, Independent Laboratory of Behavioral Studies, Lublin, Poland
Institute of Toxicology Research, Borowa, Poland
Wroclaw University of Environmental and Life Sciences Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wroclaw, Poland
Department of Forensic Medicine, Wroclaw Medical University, Poland
Department of Forensic Medicine, Wroclaw Medical University, Poland
Publikacja: 11.2022
Otrzymano: 28.09.2022
Zaakceptowano: 08.11.2022
Status artykułu: Otwarte
Licencja: CC-BY-NC-SA
Udział procentowy autorów:
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AngielskiLiczba wyświetleń: 542
Liczba pobrań: 555
Sugerowane cytowania: Nature